Give vg surject opinions about paired-end input - #5021
Merged
Conversation
This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Add this suggestion to a batch that can be applied as a single commit.This suggestion is invalid because no changes were made to the code.Suggestions cannot be applied while the pull request is closed.Suggestions cannot be applied while viewing a subset of changes.Only one suggestion per line can be applied in a batch.Add this suggestion to a batch that can be applied as a single commit.Applying suggestions on deleted lines is not supported.You must change the existing code in this line in order to create a valid suggestion.Outdated suggestions cannot be applied.This suggestion has been applied or marked resolved.Suggestions cannot be applied from pending reviews.Suggestions cannot be applied on multi-line comments.Suggestions cannot be applied while the pull request is queued to merge.Suggestion cannot be applied right now. Please check back later.
Changelog Entry
To be copied to the draft changelog by merger:
vg surjectnow requires either-i/--interleavedor-U/--force-unpairedif it thinks the input smells like paired-end data.vg giraffenow uses the GAM fragment length distribution built-in field, likevg map, instead of a"fragment_length_distribution"annotation.Description
This should help with #4563 by making it impossible to accidentally surject paired-end reads as single-ended, and by warning the user if the default unlimited fragment length limit is used.
This will not help you if you forget to map your reads as paired-end, because it specifically doesn't check for things like
/1and/2in your read names, but we expect users to be wise to that subtlety already if they have experience with linear-reference mapping tools. If we want to complain about mapping reads wrong, we should separately add that check to the mappers.