Fluorescence Imaging Laboratory, Kyiv, Ukraine
| Parameter | Value |
|---|---|
| Detection objective | Olympus PlaN 10x 0.25 |
| Magnification | 5.56x |
| Camera | Basler Ace 2R Pro (a2A5320-23umPRO), 5320x3032 px (16.1 Mpx) |
| Pixel size | 0.493 μm/px |
| Field of view | 2.62x1.49 mm |
| Z-stack step resolution | 16 μstep/μm (0.0625 μm/μstep) |
| Lateral resolution, FWHM theoretical/estimated | 1.12 μm/2.06 μm |
| Axial resolution, FWHM theoretical/estimated | 11.70 μm/ 6.32 μm |
| Excitation lasers | 510 nm, 640 nm |
| Emission filters | Chroma HQ545/40m, Chroma D620/20m |
| Parameter | Value |
|---|---|
| Detection objective | Olympus PlaN 10x 0.25 FN 22 (F18) |
| Detection tube lens | F100 |
| Magnification | ~5.56x |
| Camera | Basler Ace 2R Pro (a2A5320-23umPRO) |
| Pixel size | 0.493 μm/px |
| Field of view | 2.62x1.49 mm |
| Theoretical resolution (500 nm) | 1.220 μm |
| Theoretical depth of field (500 nm, n=1.33) | 10.640 μm |
| Z-stack step (Sutter Instruments MPC-200 with MP225/M) | 0.0625 μm/μstep (16 μstep/μm) |
| Model | Basler Ace 2R |
|---|---|
| ID | a2A5320-23umPRO |
| Sensor | Sony IMX542 (CMOS) |
| Sensor size | 14.58x8.31 mm (1.1") |
| Sensor diagonal | 16.78 mm |
| Pixels (HxV) | 5320x3032 (16.1 Mpx) |
| Pixel size | 2.74x2.74 μm |
| Frame rate | up to 24 fps |
| Pin | Function | I/O Line | Colour |
|---|---|---|---|
| 1 | 12–24 VDC camera power | - | Brown |
| 2 | Opto-coupled I/O input line | Line 1 | White |
| 3 | Ground for opto-coupled I/O line | - | Blue |
| 4 | General purpose I/O (GPIO) line | Line 2 | Black |
| 5 | General purpose I/O (GPIO) line | Line 3 | Grey |
| 6 | Ground for camera power and General Purpose I/O (GPIO) lines | - | Pink |
Pylon Viewer v7.2 camera parameters, category Digital I/O Control:
- Line Selector: Line 2
- Line Inverter: not selected
- Line Mode: Output
- Line Source: Acquisition Active
Synchronisation with Arduino32bitBoards, specs for ESP32:
- Baudrate: 115200
- Laser TTL trigger module requires 5V, need level conversion 3.3V -> 5V
Pinout:
- Trigger: Pin 5 - camera Line 2 input
- Channel 1: Pin 25/DAC - Level Converter ON
- Channel 2: Pin 26/DAC - 510 nm trigger
- Channel 3: Pin 27/PWM - 640 nm trigger
- Channel 4: Pin 15/PWM
- Channel 5: Pin 14/PWM
- Channel 6: Pin 4/PWM
- Channel 7: Pin 23/PWM
- Channel 8: Pin 19/PWM
Switch states
| Laser | Pin Hight | State |
|---|---|---|
| 510 nm | 25+26 (Ch.1+Ch.2) | 3 |
| 640 nm | 25+27 (Ch.1+Ch.3) | 4 (not working) |
| 510 nm + 640 nm | 25+26+27 (Ch.1+Ch.2+Ch.3) | 6 (not working) |
This open-source project is released under the CERN Open Hardware License. Our aims are to promote open scientific hardware development and to share our engineering solutions.