CRF_Screen

Contributors:

Runqiang Chen: Conducted in vivo screen
Megan Fridrick: Conducted in vitro screen
Huitian Diao (Yolanda): Binformatics
Matthew Pipkin: Advisor

Manuscript progress

1. Chromatin remodeling atlas: GSuite; Dropbox
2. Adam's Mll manuscript: Dropbox

Data analysis description

• In vitro analysis
• In vivo analysis

External data storage

• In vitro screen analysis:
  [Ishtar folder](/Volumes/pipkinlab/Lab Members/Former members/Megan Frederick/MAF Screen Analysis)
• In vivo screen analysis:
  Drop box link
• Raw data:
  
  • In vitro:
    [Ishtar folder](/Volumes/pipkinlab/Lab Members/Former members/Megan Frederick/MAF Screen Data)
    
  • In vivo:
    Ishtar folder
    Ishtar folder

Experiment design

• In vitro screen:
  

Fluorephore	Panel 1	Panel 2
AF700	CD44	n.a.
PerCP-Cy5.5	CD25	Lag3
BV605	CD62L	PD1
PE-Cy7	CXCR3	n.a.
APC	CD103	n.a.
PE	CD127	Tim3

• In vivo screen:
  Exp35 barcode
  Exp56 barcode
  Target constructs
  Control constructs
  

Fixed Glitches

In vitro screen - Missing wells

1. InVitro/Megan_originaldata/Screen_markers/Screen3_panel1_10U/16 10U p1.csv
   Fixed shifted cells: G10 G11 left shifted 2 cells
2. InVitro/Megan_originaldata/Screen_markers/Screen3_panel1_100U/19 100U p1.cs
   Fixed shifted cells: G10 G11 left shifted 2 cells
3. InVitro/Megan_originaldata/Screen_markers/Screen2_panel1_10U/14 10U p1.csv
   The missing wells are actually B2 - B8 instead of G5 - G11

In vivo screen - Miss placed constructs

1. Conflict references:
   Incorrect refrence:
   /InVivo/originalAnlysis/CR in vivo Replicate 2/shRNA Pools.xlsx
   Correct refrence:
   Dropbox folder

References:

1. BioGrid protein interaction
2. Human-Chromatin-Related Protein Interactions Identify a Demethylase Complex Required for Chromosome Segregation

Species	Taxonomy ID
Homo sapiens	9606
Mus musculus	10090
Rattus norvegicus	10116