I tried running the tool on a cram file (format 3.1) and got
RustQC v0.2.1 (c868466, built 2026-04-09T12:09:14Z)
Binary: x86-64-v4 (AVX-512) | CPU: AVX-512 AVX2 SSE4.2 POPCNT detected
Input: NIST7035_TAAGGCGA.marked.cram
Annotation: gencode.v28.annotation.gtf
Reference: GRCh38.primary_assembly.genome.fa
Stranded: unstranded
Paired: true
CPU Threads: 8
Output dir: ./NIST7035_TAAGGCGA
Parsing GTF annotation...
Processing NIST7035_TAAGGCGA.marked.cram
[WARN rustqc::rna::dupradar::counting] Alignment index not found for NIST7035_TAAGGCGA.marked.cram; falling back to single-threaded processing. Create an index w
ith 'samtools index' to enable parallel processing.
[E::cram_next_slice] Slice decode failure
✗ error: Failed to process NIST7035_TAAGGCGA.marked.cram: Error reading alignment record: truncated record in SAM/BAM/CRAM file
I tried running the tool on a cram file (format 3.1) and got
RustQC v0.2.1 (c868466, built 2026-04-09T12:09:14Z)
Binary: x86-64-v4 (AVX-512) | CPU: AVX-512 AVX2 SSE4.2 POPCNT detected
Input: NIST7035_TAAGGCGA.marked.cram
Annotation: gencode.v28.annotation.gtf
Reference: GRCh38.primary_assembly.genome.fa
Stranded: unstranded
Paired: true
CPU Threads: 8
Output dir: ./NIST7035_TAAGGCGA
Processing NIST7035_TAAGGCGA.marked.cram
[WARN rustqc::rna::dupradar::counting] Alignment index not found for NIST7035_TAAGGCGA.marked.cram; falling back to single-threaded processing. Create an index w
ith 'samtools index' to enable parallel processing.
[E::cram_next_slice] Slice decode failure
✗ error: Failed to process NIST7035_TAAGGCGA.marked.cram: Error reading alignment record: truncated record in SAM/BAM/CRAM file