diff --git a/packages/vite/src/node/__tests__/utils.spec.ts b/packages/vite/src/node/__tests__/utils.spec.ts
index 7974ab754301da..5d39ccbb75ee00 100644
--- a/packages/vite/src/node/__tests__/utils.spec.ts
+++ b/packages/vite/src/node/__tests__/utils.spec.ts
@@ -26,6 +26,7 @@ import {
   numberToPos,
   posToNumber,
   processSrcSetSync,
+  removeTimestampQuery,
   resolveHostname,
   resolveServerUrls,
 } from '../utils'
@@ -195,6 +196,33 @@ describe('injectQuery', () => {
   })
 })
 
+describe('removeTimestampQuery', () => {
+  test('removes timestamp query parameter', () => {
+    expect(removeTimestampQuery('/foo.js?t=1712345678901')).toBe('/foo.js')
+    expect(removeTimestampQuery('/foo.js?t=1712345678901&bar=1')).toBe(
+      '/foo.js?bar=1',
+    )
+    expect(removeTimestampQuery('/foo.js?bar=1&t=1712345678901')).toBe(
+      '/foo.js?bar=1',
+    )
+    expect(removeTimestampQuery('/foo.js?bar=1&t=1712345678901&baz=2')).toBe(
+      '/foo.js?bar=1&baz=2',
+    )
+  })
+
+  test('does not strip params with names ending in hyphen-t', () => {
+    expect(removeTimestampQuery('/foo.js?current-t=1712345678901')).toBe(
+      '/foo.js?current-t=1712345678901',
+    )
+    expect(removeTimestampQuery('/foo.js?my-t=1712345678901&other=1')).toBe(
+      '/foo.js?my-t=1712345678901&other=1',
+    )
+    expect(removeTimestampQuery('/foo.js#t=1712345678901')).toBe(
+      '/foo.js#t=1712345678901',
+    )
+  })
+})
+
 describe('resolveHostname', () => {
   test('defaults to localhost', async () => {
     const resolved = await getLocalhostAddressIfDiffersFromDNS()
diff --git a/packages/vite/src/node/utils.ts b/packages/vite/src/node/utils.ts
index a501e50eda397b..b872ea8d26c41c 100644
--- a/packages/vite/src/node/utils.ts
+++ b/packages/vite/src/node/utils.ts
@@ -338,9 +338,9 @@ export function injectQuery(url: string, queryToInject: string): string {
   return `${normalizedFile}?${queryToInject}${postfix[0] === '?' ? `&${postfix.slice(1)}` : /* hash only */ postfix}`
 }
 
-const timestampRE = /\bt=\d{13}&?\b/
+const timestampRE = /(\?|&)t=\d{13}(?:&|$)/
 export function removeTimestampQuery(url: string): string {
-  return url.replace(timestampRE, '').replace(trailingSeparatorRE, '')
+  return url.replace(timestampRE, '$1').replace(trailingSeparatorRE, '')
 }
 
 export async function asyncReplace(